RealHelix™ qPCR Kit [Green] is designed to perform a rapid real-time quantification of target DNA using a double strand DNA-binding green fluorescent dye. The convenient 2x premix contains hot-start PCR enzyme, green fluorescent dye, dNTPs, buffers, Mg²⁺, and stabilizing agent. The hot-start PCR enzyme provides high specific amplification of target DNA and minimizes the side products such as primer dimers. The separately supplied ROX dye could be used as a passive reference dye in real-time PCR instruments that are compatible with the evaluation of the ROX signal.

Figure 1. The accuracy of real-time PCR using RealHelix™ qPCR Kit [Green].

Human genomic DNAs (10, 1, 0.1, and 0.01 ng) isolated from whole blood were analyzed using the alpha 1-Antitrypsin (AAT) DNA-specific primer set and an intercalating dye. The accuracy of real-time PCR is demonstrated by E=101.7 %, R² value=0.999. Performed on the CFX96 real-time PCR detection system (Bio-Rad).

Figure 2. Comparison of real-time PCR using RealHelix™ qPCR Kit [Green] and the previous version (RealHelix™ qPCR Kit [Intercalator type]).

Human genomic DNAs (100, 10, 1, and 0.1 ng) isolated from whole blood were analyzed using a beta-globin gene-specific primer set and an intercalating dye. Performed on the CFX96 real-time PCR detection system (Bio-Rad).

Storage

• Store below -20 ℃
• Should be stored in a dark place.

Shelf life

• 12 months